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Gas Chromatography GC
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An education video from the Royal Society of Chemistry on gas chromatography using a flame ionisation detector (FID) with a brief mention of gas chromatography mass spectrometry (GCMS). From the "Modern Instrumental Techniques for schools and colleges" DVD. For more information on the Chemistry... More
Added Sep 27, 2008
Channel Education
Duration 5:17 | views 151099
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Youtube Comments 60
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Tags gas chromatography mass spectrometry chemistry royal society of rsc purification separation analysis cfof for our future education practical science spectra stem
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1BULLYSON Says:
I'm only doing this for GCSE and yet I still understand it! And it helped me, thanks!
shazzTHEpimpngturtle Says:
Polarity is definitely a factor but, another prominent factor would be the Mr of the substance, as lower Mr would constitute for lower inter-molecular forces and interactions between the components and the stationary phase and higher Mr would constitute for more. More interaction means larger Retention and hence this is why we see a small compound (Ethanol) pop out first (CH3OH) and see a larger compound (Methyl-benzene) pop out second (C7H8).
Tougain Says:
This has nothing to do with polarity, you maybe talking about HPLC in GLC the more volatile gas are the one that have a boiling point lower than the oven so they remain as gas some components are soluble in the stationary phase so remain less in the helium gas that's why it is known as partition not adsorption adsorption we can talk about polarity, but here we are talking about phase.
BuickDoc Says:
One method I used is called preparative gc. A larger column and a larger volume of injectate is used. As the carrier gas exits the machine, dry ice cooled traps are inserted in the gas stream. Any liquids are thus trapped. Change the trap for each peak on the chromatogram, to isolate each compound. They can then be identified by standard methods, ie IR, NMR, Mass Spec, etc. Another method is to use a GC/Mass Spectrometer. High resolution MS will usually give a unique structure.
callmehoo Says:
Hello. I want to ask about GC TCD-thermal conductivity detector can u show a video about it?
callmehoo Says:
If a liquid is viscous that implies that the liquid has resistance to flow. For example petroleum gel and water, which is more viscous? The answer is petroleum gel as it flows more slowly than water across a slanted plane.
ncpatch Says:
Any one know what viscous means?
bonny247 Says:
hii friends can any one give me information on what compounds can be dervitized??? and what is the exact method for sample extraction with the use of derivatizing reagents?? lidocaine, procaine mesalamine bupivacaine topermate can any of these be dervatized??
crudeoilsystems Says:
very nice. Thanks
hplcflowmeter Says:
thanks
shortie177 Says:
you need to use the external, internal standard or standard addition method of anlysis to determine which peak is which if you have unknowns.
shaggyburns Says:
how do you know which compound each peak is?
asteriscotriangulado Says:
Awesome! :D
Yibitech Says:
The components are sticking and being released LOTS of times. That stickiness has to do with the polarity of the column, and the polarity of the sample. Volatility is ease of evaporation. The particles are sticking to the coating on the stationary phase, coming off, and sticking again. Like dissolves like, so a polar chemical will stick and release to a polar column more times, thus coming off the column slower.
angelzpoo Says:
I didn't think you had to use the detector? Isn't it destructive? The GCMS does not have a detector because it is the ms aspect does that job??? is the gcms form of testing destructive?
angelzpoo Says:
I didn't think you had to use the detector? Isn't it destructive?
analychemist Says:
GC detects relative amounts of some analyte. To find concentrations, usually you do the method of internal standards. You compare the area ratio between what you want as an analyte and some standard which has a fixed concentration of your choice. You make standards of your analyte (using known amounts of the analyte) with the standard to make a calibration curve. From the curve and running your unknown, you can find concentration. That's probably more than what you wanted lol.
Jerby7 Says:
Hello, Does the GC detects a concentration or a number of mole? I hope my question is clear enough to understand
Jerby7 Says:
thanks for sharing!
nbo345 Says:
the sample is heated quickly after it is injected into the injection port where it is vaporized then carried to the (nonpolar or polar) column.(Preferably, nonpolar column are used to prevent bleeding) This also regards the polarity of the sample and the column wall. Organic or inorganic samples can be tested. In this case protein, it must be homogenized into liquid form before using GC. Other parameters also play a factor in the identification of analytes in the sample
goalone09 Says:
thank you very much.
Sarimperil Says:
Very informative and easy to understand! Thank you!
farahfd20 Says:
very helpful video
vamsipalaka Says:
thank u very much.
Ezequiel9425 Says:
thanks